Principles and Techniques of Electron Microscopy. Biological Applications. Volume 2
نویسنده
چکیده
In the relatively short time since its introduction, the technique of electron microscopy has tended to acquire an aura of mystique such that the process of specimen preparation for the electron microscope and the production of a micrograph from this may be compared with an inflexible religious ceremony. Dr. Hayat's book, together with the first volume in this series, should do much to dispel this air of mysticism. The present volume has chapters on freeze substitution and freeze drying, freeze etching, negative staining, shadow casting and replication, high resolution and shadowing, and autoradiography. Each chapter considers theoretical aspects, practical aspects and interpretation of results obtained with each technique. Emphasis is placed on preparative procedures and equipment so that each of the techniques can be reproduced. In many of these approaches interpretation of data and the production of artefacts are serious problems and these are considered in detail, especially in the chapters on freeze etching, shadowing, and negative staining, where experience is essential to interpret electron images. All chapters are fairly well illustrated with regard both to commonly found artefacts and to image interpretation. In general the volume fulfills the aim of the editor and should provide a practical guide for the application of these techniques for electron microscopists. With the increased emphasis of these aspects of electron microscopy, for example, freeze etching in membrane studies and autoradiography in biochemistry and physiology, it should also be of value to scientists of varied disciplines.
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ورودعنوان ژورنال:
- The Yale Journal of Biology and Medicine
دوره 47 شماره
صفحات -
تاریخ انتشار 1974